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Bioinformatics Software: Partek Genomics Suite (PGS)

General Information

NML Bioinformatics is very pleased to host a two-day hands-on training event for Partek Flow and Partek Genomics Suite (PGS).

Major Topics:

  • Next-gen sequencing data analysis
    • RNA-seq analysis with Partek Flow
    • DNA-seq analysis with Partek Flow
  • Microarray data analysis
    • Gene expression analysis
  • Data visualization
    • PCA, heatmap, hierarchical clustering, dot plot, volcano plot, interaction plot, etc.)

Key Features:

  • Hands-on practices:
    Step-by-step instructions on analyzing microarray and next-generation sequencing data
  • Professional consultation:
    Bring your own data and go through analysis with the guidance of the Partek customer support team

Time: Nov.4-5, 2015

Place: Aresty Auditorium, HSC

Registration is mandatory. 

Training Agenda

Wednesday Nov.4, 2015

8:45am – 9:00am: Breakfast (provided)

9:00am – 12:00pm: RNA-seq Data Analysis with Partek Flow

  • Import fastq files
  • Pre-alignment QC
  • Trim reads
  • Alignment
  • Post-alignment QC
  • Quantification
  • Differential expression detection
  • Visualization (PCA, hierarchical clustering, dot plot, chromosome view, volcano plot etc)
  • GO enrichment analysis

12:00pm – 1:15pm: Lunch (provided)

1:15pm – 4:00pm: DNA-seq Data Analysis with Partek Flow

  • Import bam files
  • Variant detection
  • Variant annotation
  • Variant report and visualization

Thursday Nov.5, 2015

8:45am – 9:00am: Breakfast (provided)

9:00am – 12:00pm: Microarray Data Analysis with Partek Genomics Suite

  • Import .cel files in PGS
  • Perform QA/QC of imported data
  • Detect differential expression (ANOVA)
  • Gene list creation
  • Biological interpretation
  • Visualization (PCA, histogram, dot plot, volcano plot, interaction plot, heatmap etc.)

12:00pm – 1:30pm: Lunch (on your own)

1:30pm – 4:00pm: Q&A and Open Lab

  • Bring your own data